Isolation and Culture of Neonatal Murine Primary Cardiomyocytes

The cardiomyocyte is the main cell type in heart responsible for its contractile function. Culturing primary cardiomyocytes from mammalian sources to study their function remains challenging as they are terminally differentiated and cease multiply soon after birth. major technical hurdles associated with culture include attaining high yields, obtaining healthy/viable cells that show spontaneous contractions upon culture, avoiding contamination by non-myocyte cardiac types such fibroblasts endothelial cells. yield quality of obtained impacted a variety factors, purity reagents, composition digestion mixture, conditions, temperature tissue during different steps isolation. Here, we provide simplified workflow isolate, maintain neonatal rats/mice dishes, which can then be used study, instance, hypertrophy drug-induced cardiotoxicity. © 2021 Wiley Periodicals LLC. Basic Protocol: Isolation rat/mouse pups Support Coating plates extracellular matrix substrates efficient attachment